This study evaluated the performance of the
BD Phoenix system for the identification (ID) and antimicrobial susceptibility testing (AST) ofStaphylococcus
vitulinus. Of the 10 S. vitulinusisolates included in the
study, 2 were obtained from the Czech Collection of
Microorganisms, 5 from the environment, 2 from human
clinical samples, and 1 from an animal source. The results
of conventional biochemical and molecular tests were used
for the reference method for ID, while antimicrobial susceptibility testing performed in accordance with Clinical
and Laboratory Standards Institute recommendations and
PCR for themecAgene were the reference for AST. Three
isolates were incorrectly identified by the BD Phoenix
system; one of these was incorrectly identified to the genus
level, and two to the species level. The results of AST by
the BD Phoenix system were in agreement with those by
the reference method used. While the results of susceptibility testing compared favorably, the 70% accuracy of the
Phoenix system for identification of this unusual staphylococcal species was not fully satisfactory.
This study evaluated the performance of theBD Phoenix system for the identification (ID) and antimicrobial susceptibility testing (AST) ofStaphylococcusvitulinus. Of the 10 S. vitulinusisolates included in thestudy, 2 were obtained from the Czech Collection ofMicroorganisms, 5 from the environment, 2 from humanclinical samples, and 1 from an animal source. The resultsof conventional biochemical and molecular tests were usedfor the reference method for ID, while antimicrobial susceptibility testing performed in accordance with Clinicaland Laboratory Standards Institute recommendations andPCR for themecAgene were the reference for AST. Threeisolates were incorrectly identified by the BD Phoenixsystem; one of these was incorrectly identified to the genuslevel, and two to the species level. The results of AST bythe BD Phoenix system were in agreement with those bythe reference method used. While the results of susceptibility testing compared favorably, the 70% accuracy of thePhoenix system for identification of this unusual staphylococcal species was not fully satisfactory.
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This study evaluated the performance of the
BD Phoenix system for the identification (ID) and antimicrobial susceptibility testing (AST) ofStaphylococcus
vitulinus. Of the 10 S. vitulinusisolates included in the
study, 2 were obtained from the Czech Collection of
Microorganisms, 5 from the environment, 2 from human
clinical samples, and 1 from an animal source. The results
of conventional biochemical and molecular tests were used
for the reference method for ID, while antimicrobial susceptibility testing performed in accordance with Clinical
and Laboratory Standards Institute recommendations and
PCR for themecAgene were the reference for AST. Three
isolates were incorrectly identified by the BD Phoenix
system; one of these was incorrectly identified to the genus
level, and two to the species level. The results of AST by
the BD Phoenix system were in agreement with those by
the reference method used. While the results of susceptibility testing compared favorably, the 70% accuracy of the
Phoenix system for identification of this unusual staphylococcal species was not fully satisfactory.
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