3.2. IAC
The IACwas constructed with the primer pair of prs-F, R to verify
the false-negative PCR results and co-amplified with the Listeria
genus specific primer pair resulting in 100-bp (IAC) and 370-bp
(Listeria genus) products. A PCR was performed to determine the
detection limit using constructed IAC ranging from 1 to 15 ng. The
detection limit was achieved at 5 ng of IAC with the presence of
150 ng of Listeria genomic DNA with 35 cycles of amplification.