This experiment was designed to evaluate the activity of the extract or isolated compound(s) against a plant fungal pathogen. In the first experiment, we determined the in vitro efficacy of the solvent and the crude extract and amphotericin B. After four days of incubation, the acetone treatment led to the highest growth (or lowest inhibition) with a growth diameter of 5.7 to 6.0 mm in all cases. It appeared not to inhibit the growth of the three Penicillium species. This confirms the low level of toxicity of acetone to fungi ( Eloff et al., 2007). Acetone appears not to be harmful to the fungi or had evaporated to such an extent that the residues left had no effect ( Table 2). The observations were repeated after seven days but with the exception of some growth of P. expansum dosed with 10 mg/ml acetone extract, there were no striking differences in the values between 4 and 7 days