But based on the above results YCA has optimum tolerance in a wide range up to 14%. RAPD analysis showed the different monomorphic and polymeric bands among these strains and from the dendrogram analysis the 7 strains were divided in to two groups and further in to sub clusters. These studies prove that the substrates have a major impact on the S. cerevisae and can induce some change in the genotype which tends to develop in to different strains. The polymorphism found in these strains may be the reason for this type of results which was yet to be confirmed by further studies.