An aliquot of the final solution (1 mL) was filtered through 0.45 lm membrane filters from Millipore (Brazil) using a plastic syringe with a capacity of 10 mL
of Plascalpand and transferred to a vial with capacity of 1.5 mL of
Uniglas and then injected into the chromatograph. The concentration
of each sample was determined by HPLC–PAD