The dried extracts were reconstituted in HPLC grade methanol
and spiked with an internal standard, 10 pmol of N-arachidonoylglycine-d8. An Agilent 1260 liquid chromatography system with a Kinetex™ 2.6 lm C18 100 Å (50 2.1 mm) reverse phase column
was used for the separation. Mobile phase A consisted of water with 0.1% formic acid and mobile phase B consisted of acetonitrile
with 0.1% formic acid