The minced sample (1 g) was
homogenised with 10 ml deionised water and an aliquot of the
sample (1 ml) was added to 2 ml of trichloroacetic acid/thiobarbituric
acid (TCA/TBA) stock solution consisting of 15% TCA (w/v) and
0.375% TBA (w/v) in 0.25 M HCl and 3 ml 2% butylated hydroxytoluene
(BHT) (w/v) prepared in absolute ethanol in a test tube and
immediately mixed thoroughly with a vortex mixer.