temperature 26ºC±1ºC, a photosynthetic proton flux
density (PPFD) of 200 μmol m-2 s-1 and 14/10 h
photoperiod and sub-cultured at intervals of 20 days
to the same medium variants. Well-developed
shoots (2-3 cm) were isolated from explants and
transferred on rooting medium consisting of ½ MS0
and 0.1 mgL-1 IAA.
The experimental design for regeneration was:
three replication with 20 explants of each, twice,
120 explants per treatment – culture medium
variant, explant type and genotype. The
callusogenesis, organogenesis and regeneration
frequency (% explants with response) and number
of regenerants per explant (compared to reacted
with organogenesis explants) were examined for a
period of 90 days.