The whole
collection was shown to be able to grow at 30 and 10°C
in liquid whole egg (Jan et al. 2011). The present study
shows that 50% of the collection (34 isolates) was also
able to grow at 6°C in liquid whole egg, reaching the
same final level of population at both refrigerated temperatures
(around 78 log CFU ml
1 at 6 and 10°C,
Table 1). Under these conditions, the average final cell
concentration was higher in liquid whole egg than in optimum medium (around 78 vs 69 log CFU ml
1 in
liquid whole egg and in BHI-YE, respectively). Ninety-six
percents of the collection (65 isolates) was also shown to
be able to spoil liquid whole egg at 30 and 10°C, with
onsets of egg spoilage varying from 113 to 36 h and
from 5 to 24 days at 30 and 10°C, respectively. Only
three isolates (isolates Nr35, Nr41 and Nr47) did not
spoil liquid whole egg, neither at 30°C nor at 10°C, even
after 2 months incubation (results of this study in
Table 1).