This study has demonstrated the optimization potential of silk
scaffolds in terms of structure (porosity, pore dimensions and pore
geometry) and biomechanics for bone tissue engineering applications.
We have demonstrated that human adipose-derived stem
cells interpret the extracellular environment, by responding differently
to the architecture of silk scaffolds and producing bone-like
extracellular matrix in a manner that appears to depend on the
structure and stiffness of the scaffolds. Based on the collected data,
we have proposed a conceptual model that correlates bone tissue
formation with the architecture and stiffness of silk scaffolds,
which emphasizes the importance of appropriate scaffold design
when engineering bone.