Effect of Different Incubation Times
The bacterial isolate J1 was grown in
conical flask (250 ml) with 100 ml sucrose
/yeast extract broth medium and adjust pH
at 7.0 were sterilized at 121
oC for 20 min
The inoculated flasks were incubated at
30oC at 150 rpm under different incubation
times (24, 48, 72, 96 hr.).After 48 hr., PHB
produced were quantified.