It was run at
300 μL min−1 starting with 100% component A at injection
time and gradually changing to 60% A (40% B) over
3 min, and it was then changed to 10% A (90% B) until
22 min after injection. This eluent composition was kept for
8 min and then shifted within 0.1 min back to the starting
conditions (100% component A) and kept there until
38 min after injection.