During micropropagation the exudation of phenol is very common
and it often influences the result. In Aristolochia indica leaching of
polyphenols affected the shoot growth in vitro. However, addition of AC
in the medium as well as increased subculturing frequency reduced this
problem considerably (Soniya and Sujitha, 2006). Manjula et al. (1997)
noted that 0.05 g/l AC was sufficient to reduce polyphenol exudation
from A. indica. The polyphenols associated with Sorghum (Sorghum
bicolour) tissue culture resulted in blackening of inoculated tissues
within a day and this can result in tissue necrosis and, in severe cases,
explant death also