Highest TCA-soluble peptides in clown featherback corresponded to the highest degradation of MHC as observed in SDS-PAGE (Fig. 2B).The highest degradation of MHC was observed at pH 4 and 7 as shown by the lowest band intensity and actin band was reconfirm that as a a substrate for proteolytic enzymes, but wasn’t preferable when compared with MHC of clown featherback by band intensity.