Fig. 1. In vitro regeneration of nodal explants derived from mature tree of
H. opposita. (A) Nodal explants with multiple shoot development on MS
medium supplemented with 4.4 μMBA after 30 days of culture. (B) Stunted and
malformed shoots developed on MS medium supplemented with 4.7 μM Kin
after 30 days of culture. (C) Development of roots from the base of an in vitro
regenerated shoot on MS medium supplemented with 3.6 μMIAA after 21 days.
(D) Three-month-old regenerated plants in the greenhouse.