Sea grass is spreading in tropical and subtropical areas. Seagrass is vital to coastal ecosystems is enormous. The seagrass serves as habitat for aquatic life an embryo And is the primary food for fish. Turtle dugong And on the ecology of sea grass also a source of food for animals with economic value The objective of this study. To examine the genes of six species of sea grass. Halodule pinifolia, Enhalus acorides, Cymodocea serrulata, Halodule uninervis, Halophila ovalis and Thalassia hemprichii. They used a standard gene and gene rbcL, matK standard verification technique PCR. The results showed that DNA has occurred approximately 700-800 base pairs 900-1000 bp, respectively. The genes detected in the sea grass. Measures consistent with other plants. Which can be used to develop a DNA barcode. In order to identify or specify the species and sea grass.