Real-time PCR were performed with the BioRad CFX96 Touch (BioRad, USA) using optical grade plates. The PCR reaction was performed on a total volume of 25 μl using the iQTMSYBR Green Supermix (BioRad, USA).
Real-time PCR were performed with the BioRad CFX96 Touch (BioRad, USA) using optical grade plates. The PCRreaction was performed on a total volume of 25 μl using the iQTMSYBR Green Supermix (BioRad, USA).