Results and discussion
A. tumefaciens is known for its T-plasmid that capable to transfer desired gene to plants. Glucuronidase gene is
capable to convert X-gluc (5-bromo-4-chloro-3-indolyl glucuronide) substrate into glucuronic acid and proceeded
by oxidation dimerization it will turn into 5.5’-dibromo-4.4’-dichloro-indigo, a compound that coloured blue. The
GUS assay result showed that the cotyledon turned blue which mean glucuronidase gene can be transferred into J.
curcas genome (Fig. 1).