According to the Cosmetics Regulation (European Commission, 2009), it is prohibited in the EU to market cosmetics products and their ingredients if they have been tested on animals for most human health effects, including acute toxicity. This imposes on the cosmetics industry the need for alternative approaches to the safety testing of the 105 ingredients of consumer products. After a meeting of experts organised by the European Centre for the Validation of Alternative Methods (ECVAM), the alternative methods that existed at the time and had been applied to cosmetics were reviewed (Adler et al., 2011, Hartung et al., 2011).