Effects of endophytic actinomycetes and Myanmar Bradyrhizobia on
growth, nodulation, nitrogen fixation and seed yield of three recommended
soybeans from Myanmar (Hinthada), Thailand (SJ 5) and Cambodia (DT 84)
were studied in pot experiments at Chiang Mai University during November,
2008 to March 2009. Sterile soil by autoclaving was used for cultivation under
open field using tap water. In each pot trial, one soybean variety was tested,
using randomized complete block experimental design with 3 replications and
6 treatments. The tested treatments were as follows: uninoculated control
treatment, two Bradyrhizobial inoculated treatments with MA and MB,
Bradyrhizobial isolates from Myanmar, single inoculated treatment with a
selected endophytic actinomycetes, EA (Streptomyces sp.) and two dual inoculated
treatments with EA+MA and EA+MB. N2 fixations of soybean plants were
evaluated by ureide technique, using root bleeding sap. We found out that the
tested treatments had no significant effects on nodule dry weight of the tested
soybean varieties at both V6 and R3.5 growth stages. Single inoculation with
MB resulted in significant improvement of shoot N uptake at R3.5 stage, amount
of seasonal fixed N and seed yield of all tested soybean varieties compared to
uninoculated control but MA was effective only for Cambodian soybean. Single
inoculation with EA had no significant effects on all studied parameters and dual
inoculation with EA+MB even showed significant depressive effect on amount
of seasonal fixed N of all soybean varieties compared with single inoculation
with MB treatment. The effects of dual inoculation with EA and MA on all
studied parameters were not significant but this treatment tended to be more
effective than single inoculation with MA.
Effects of endophytic actinomycetes and Myanmar Bradyrhizobia ongrowth, nodulation, nitrogen fixation and seed yield of three recommendedsoybeans from Myanmar (Hinthada), Thailand (SJ 5) and Cambodia (DT 84)were studied in pot experiments at Chiang Mai University during November,2008 to March 2009. Sterile soil by autoclaving was used for cultivation underopen field using tap water. In each pot trial, one soybean variety was tested,using randomized complete block experimental design with 3 replications and6 treatments. The tested treatments were as follows: uninoculated controltreatment, two Bradyrhizobial inoculated treatments with MA and MB,Bradyrhizobial isolates from Myanmar, single inoculated treatment with aselected endophytic actinomycetes, EA (Streptomyces sp.) and two dual inoculatedtreatments with EA+MA and EA+MB. N2 fixations of soybean plants wereevaluated by ureide technique, using root bleeding sap. We found out that thetested treatments had no significant effects on nodule dry weight of the testedsoybean varieties at both V6 and R3.5 growth stages. Single inoculation withMB resulted in significant improvement of shoot N uptake at R3.5 stage, amountof seasonal fixed N and seed yield of all tested soybean varieties compared touninoculated control but MA was effective only for Cambodian soybean. Singleinoculation with EA had no significant effects on all studied parameters and dualinoculation กับ EA + MB แสดงผลสำคัญ depressive ยอดแม้ของ N ถาวรตามฤดูกาลของพันธุ์ถั่วเหลืองทั้งหมดเปรียบเทียบกับ inoculation เดียวด้วยการรักษา MB ผลกระทบของ inoculation คู่กับ EA และ MA ในทั้งหมดพารามิเตอร์ที่ศึกษาไม่สำคัญ แต่การรักษานี้มีแนวโน้มจะเพิ่มมากขึ้นมีประสิทธิภาพกว่า inoculation เดียวกับ MA
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