This study was carried out to determine whether butter can be a carrier for probiotics by observing the
survivability of selected probiotic strains during cold storage. The effects of using probiotic adjunct
cultures (Lactobacillus acidophilus ATCC 4356 and Bifidobacterium bifidum ATCC 29521) in butter on
microbiological counts, sensory characteristics, chemical characteristics and free fatty acid (FFA)
composition during storage for 60 days were investigated. The butter samples produced with B. bifidum
ATCC 29521 maintained the probiotic characteristics, in that the level of viable cells of the probiotic was
>106 cfu g1 until 30 days of storage. The highest scores in sensory assessment were obtained on the first
day of storage. FFAs, including C2:0, C6:0, C14:0 and C18:1, were affected significantly by storage period
and by the adjunct cultures, however conjugated linoleic acid and C18:2 were not affected by storage
period and the use of probiotic adjunct culture.