To investigate the effects of different metal ions and compounds
on -amylase activity, the purified -amylase was preincubated
for 1 h at 37 ◦C with 5 mM of various metal ions, 10 mM ethylenediaminetetraacetic
acid (EDTA), phenylmethanesulfonyl fluoride
(PMSF), Tween 80, SDS and dl-dithiothreitol (DTT), individually.
Residual activity was determined under optimal enzyme assay conditions.
Activity assayed in the absence of any additives was taken
as 100%.
To investigate the effects of different metal ions and compoundson -amylase activity, the purified -amylase was preincubatedfor 1 h at 37 ◦C with 5 mM of various metal ions, 10 mM ethylenediaminetetraaceticacid (EDTA), phenylmethanesulfonyl fluoride(PMSF), Tween 80, SDS and dl-dithiothreitol (DTT), individually.Residual activity was determined under optimal enzyme assay conditions.Activity assayed in the absence of any additives was takenas 100%.
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