Chromatographic peakswereidentifiedbasedonretention
times, massspectraofauthenticcompoundswhenavailable,
Adams (2007), NIST98andWILEY275librariesofmassspectra
and publisheddata(Adams, 2007).
2.6. Quantificationandsemi-quantificationbygas
chromatography-flame ionizationdetector(GC-FID)
Essential oilcompositionwasquantifiedusingaHewlett-
Packard 5890IIGC,equippedwithflameionizationdetector(FID)
and anon-polarcolumnRtX-5MS(30m×0.25mmi.d.×0.25m
film thickness).Thevolumeofthesamplesmanuallyinjectedwas
1L (insplitlessmode).Heliumwasusedasthecarriergaswith
a flowrateof1mL/min.Theoventemperaturewasprogrammed
in thesamewayasforGC–MSanalysis.Theinjectoranddetector
temperatures were220 ◦C and300 ◦C respectively.Quantification
was carriedoutbasedonasix-pointcalibrationcurveforeach
compound understudy.
The semi-quantificationprocedurewasperformedbycompar-
ing theareasofpeaks,inordertodeterminetheproportionof
each compoundpresentinthearomaticprofilewithrespecttothe
technique usedforisolatingtheessentialoilfromtheplanttissues.