Three species of freshwater algae (Spirulina platensis, Euglena gracilis and Chlorella vulgaris)
labeled with 14C were fed to larval tilapia (Oreochromis niloticus) with a standard length (SL) from
0.8 to 3.4 cm. This was conducted to clarify the acceptability of the three species of algae to
developing larval tilapia based on their ingestion and assimilation of the 14C-labeled algae.
Ingestion rate (IR, Ag C/fish/h) and assimilation rate (AR, Ag C/fish/h), as well as ingestion
efficiency (IE, %) and assimilation efficiency (AE, %) were investigated by monitoring the fate of
the labeled food. Larval tilapia could ingest 2.6 – 85% of the available S. platensis biomass with
larvae growing from an SL of 0.8 – 3.4 cm, and the relationship between IR and body weight (BW,
mg) was expressed as IR = 15.62BW0.686 (r
2
= 0.94). Larval tilapia ingested significantly less E.
gracilis (1.6 – 26% of the available Euglena biomass) than Spirulina, and the relationship between
IR and BW was IR = 13.70BW0.584 (r
2
= 0.93). In contrast, a negligible amount (1.6 – 20% of the
available C. vulgaris biomass) of Chlorella was ingested by larval tilapia over the range of sizes
studied. For all three algae, a relatively lower assimilation efficiency (AE) was found in larvae right
after the onset of exogenous feeding, but AE improved rapidly with increasing larval size from 0.8
to 1.2 cm SL. Spirulina was more readily assimilated (61.4 – 80%) than Euglena and Chlorella.
Assimilated carbon from Euglena and Spirulina were about 1 – 2 and 3 – 6 times that of the resting
carbon consumption requirement for respiration of the larval tilapia. It was concluded that Spirulina
Three species of freshwater algae (Spirulina platensis, Euglena gracilis and Chlorella vulgaris)
labeled with 14C were fed to larval tilapia (Oreochromis niloticus) with a standard length (SL) from
0.8 to 3.4 cm. This was conducted to clarify the acceptability of the three species of algae to
developing larval tilapia based on their ingestion and assimilation of the 14C-labeled algae.
Ingestion rate (IR, Ag C/fish/h) and assimilation rate (AR, Ag C/fish/h), as well as ingestion
efficiency (IE, %) and assimilation efficiency (AE, %) were investigated by monitoring the fate of
the labeled food. Larval tilapia could ingest 2.6 – 85% of the available S. platensis biomass with
larvae growing from an SL of 0.8 – 3.4 cm, and the relationship between IR and body weight (BW,
mg) was expressed as IR = 15.62BW0.686 (r
2
= 0.94). Larval tilapia ingested significantly less E.
gracilis (1.6 – 26% of the available Euglena biomass) than Spirulina, and the relationship between
IR and BW was IR = 13.70BW0.584 (r
2
= 0.93). In contrast, a negligible amount (1.6 – 20% of the
available C. vulgaris biomass) of Chlorella was ingested by larval tilapia over the range of sizes
studied. For all three algae, a relatively lower assimilation efficiency (AE) was found in larvae right
after the onset of exogenous feeding, but AE improved rapidly with increasing larval size from 0.8
to 1.2 cm SL. Spirulina was more readily assimilated (61.4 – 80%) than Euglena and Chlorella.
Assimilated carbon from Euglena and Spirulina were about 1 – 2 and 3 – 6 times that of the resting
carbon consumption requirement for respiration of the larval tilapia. It was concluded that Spirulina
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