Chemical marker yield increased with time over
60 min for 1.5% and 3% D-ribose while yield reached
to the saturation point around 20 min with 4.5% D-ribose
as shown in Fig. 4. This suggests that the amino
acid content in mashed potato was adequate for 1.5%,
3% D-ribose until 60 min, while amino acid was consumed
completely around 20 min in case of 4.5% D-ribose
(Fig. 4). Analytical study showed that total
amino acid content in 15 g of mashed potato is much
less compare to 1.5 g D-ribose. Hence, acid content
was observed as the limiting factor during chemical marker
M-2 formation.