This corresponds to a maximum H2 yield from starch of 1.75 mol/-
mol glucose equivalents, which is an intermediate value between
2.2 mol/mol hexose obtained under optimised conditions by Lin
and co-workers [27] and 1.19 mol/mol glucose obtained in the fermentation of pre-treated corn starch [28]. The H2 yield from
sucrose was 3.35 mol/mol, that is higher than other values
reported with mesophilic mixed cultures, such as 2.39 and
1.8 mol H2/mol sucrose [29,30], or even with a pure culture of C.
butyricum CGS5 (2.78 mol H2/mol sucrose) [31]. These results show
that in general mixed cultures can produce H2 in similar or even
higher yields than pure cultures