For isolation from waste water sample, 1 mL of water sample was added in 99 mL sterilized water. After serial dilution (10−3 to 10−7), 1mL of each dilution was spread on carbon rich nutrient agar plate.
For isolationfrom waste water sample, 1 mL of water sample was addedin 99 mL sterilized water. After serial dilution (10−3 to 10−7),1mL of each dilution was spread on carbon rich nutrientagar plate.