2.2 Start-up and operation A 10L-jacketed fermenter (Biostat B) equipped with pH probe, stirrer, sampling ports and temperaturecontroller was used in this study. The working volume of
the bioreactor was maintained at 7 Litre and ran under uncontrolled pH, which is without acid or base addition. Experiments was carried out at thermophillic temperatureof 53oC by circulating water from a thermostat through a water jacket surrounding the bioreactor, and mixing was aided by a mechanical stirrer set at a speed of 150rpm. Thesystem was started up as batch to achieve an active acidifying culture by loading the substrate seeded with POME, then sealed and purged with Nitrogen gas for 15 minutes. Semi-continuous feeding started from day 10,where a known volume of slurry was withdrawn daily from the reactor and replaced with fresh feedstock via the slurry sampling ports equivalent to an HRT of 10 days. In addition, approximately 50 ml of the sample was taken daily from the bioreactor through the sampling port, whichthen underwent series of analysis. Biogas production was measured by water displacement method.
2.2 Start-up and operation A 10L-jacketed fermenter (Biostat B) equipped with pH probe, stirrer, sampling ports and temperaturecontroller was used in this study. The working volume of
the bioreactor was maintained at 7 Litre and ran under uncontrolled pH, which is without acid or base addition. Experiments was carried out at thermophillic temperatureof 53oC by circulating water from a thermostat through a water jacket surrounding the bioreactor, and mixing was aided by a mechanical stirrer set at a speed of 150rpm. Thesystem was started up as batch to achieve an active acidifying culture by loading the substrate seeded with POME, then sealed and purged with Nitrogen gas for 15 minutes. Semi-continuous feeding started from day 10,where a known volume of slurry was withdrawn daily from the reactor and replaced with fresh feedstock via the slurry sampling ports equivalent to an HRT of 10 days. In addition, approximately 50 ml of the sample was taken daily from the bioreactor through the sampling port, whichthen underwent series of analysis. Biogas production was measured by water displacement method.
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