The FRAP (Ferric ion reducing antioxidant power) assay was
used to evaluate ferric reducing antioxidant potential of C. paniculatus
seed extracts, prepared in different solvents. FRAP reagent
was prepared by mixing 300 mM/L NaOAc (sodium acetate, pH 3.6)
buffer with 10 mM/L TPTZ (2,4,6-tripyridyl-s-triazine) solution in
40 mM HCl and 20 mM/L FeCl3·6H2O (ferric chloride solution) in
the ratio of 10:1:1 (Benzie and Strain, 1996). Freshly prepared
reagent was warmed to 37 ◦C in a water bath before use. For
performing the assay, 100 L of each extract was mixed with
3 mL freshly prepared FRAP reagent. The reaction mixture was
incubated for 4 min at room temperature. The absorbance of
reaction mixture was measured at 593. The standard curve was