Zn(II)-dependent fluorescence signal enhancement of DA-ZP1-TPP.
Normalized fluorescence spectra of a 1 μM solution of DA-ZP1-TPP in buffer
(50 mM PIPES, 1 mM EDTA, 2 mM CaCl2, and 100 mM KCl; pH 7) on increasing
concentrations of free zinc ions. (Inset) Zinc-binding isotherm normalized to
the integrated fluorescence signal intensity under zinc-saturating conditions