Tomato is a good example of a successful use of mutations affecting major genes for plant breeding however, mutant alleles are only currently known for an insignificant fraction of the about 35,000 genes in the tomato genome hence, large scale mutagenesis and introgression of natural genetic variation can be useful to fill this gap [1]. Induced
mutagen-sis as a breeding strategy for improvement of tomato has been explored through different studies [2-5]. The present investigation was undertaken to study the frequency and spectrum of macro-mutations along with mutagenic efficiency and effectiveness of gamma rays, ethyl methane sulphonate (EMS) and their combinations in three genotypes of tomato.