Production of Cellulase-Free Xylanase from a Novel Yeas Strain Used for Biobleaching in Paper Industry
Abstract
An investigation was conducted to explore the microbial xylanases that are thermostable and cellulose – free are generally preferred for biobleaching of paper pulp A novel yeast strain Pichia pinus isolated from a decaying wood produced up to 545 IU ml-1 of xylanolytic ennzymes when grown on a medium containing 4 % oat spelt xylan. The crude xylanase preparation showed no activity towards cellulolytic substrates but low levels of B-xylosidase (0.1 IUml-1 )and