Chemical Analysis. Dietary Fiber. The AOAC enzymaticgravimetric method (8) was used, modified by using dialysis
against water instead of ethanol precipitation of soluble dietary
fiber (9) for dietary fiber analysis. After enzymatic hydrolysis
of digestible components, insoluble dietary fiber (iDF) and
soluble dietary fiber (sDF) fractions were separated and
chemically hydrolyzed. The remaining residue was gravimetrically quantified as Klason lignin (KL). Constituent neutral
sugars (NSs) and uronic acids (UAs) were quantified in the
hydrolysates. UAs were quantified spectrophotometrically by
the Scott method (10). NSs were determined by gas chromatography as alditol acetates (11)