Confirmation of Monacolin K concentration in MFR95 crude extract was performed using HPLC method. By use of 200 g of MFR for extraction, the yield of Monacolin K was measured. The result showed that our MFR95 crude ethanol extract contained about 1760 g g-1 of Monacolin K. The amount of Monacolin K in solid state fermentation was influenced by the type or species of microorganism used for fermentation. We compared the concentration of Monacolin K in our MFR95 crude extract that fermented by Monascus sp. of ITB collection (Monascus purpureus HD001) with another MFR that fermented by Aspergillus sp.and Monascus sp.. The results showed that yield of Monacolin K in rice that fermented by Monascus purpureus HD001 was two folds higher than rice that fermented by Aspergillus terreus and another species of Monascus purpureus (See Table 3). However, different type of strain will also give fluctuative yield of Monacolin K content. This was because of each microorganism had its own optimum condition and need different strategy of extraction and fermentation process.