It was observed that aao activity in broccoli floret was 7 times higher than in broccoli stalks, indicating that enzymatic conversion of l-aa to dhaa could be higher in floret than in stalks. Although thermal inactivation was studied only for aao in this study, the inactivation of other l-aa oxidizing enzymes such as APx should also be investigated because their role in l-as degradation could not be excluded in this study