De-proteinized whey can be utilized as a feed stock for production of microbial oil.
" DP-whey treated with lactase resulted in the highest microbial oil concentration.
" A higher biomass and oil were obtained with higher initial lactose concentrations.
" The best kinetic parameters were determined in DP-whey treated with lactase.
" The maximum c-linolenic acid content of 5.48% was obtained at the highest lactose concentration in DP-whey without enzyme addition