Tea (Camellia sinensis (L.) O. Kuntz) hybrid seeds were obtained by crossing high yielding drought and stem canker tolerant cultivar TRI 3013 (female parent) with the cultivar DT 95 (male parent) which is moderately yielding and resistant to nematodes. Embryos of hybrid seeds were isolated and regenerated on MS medium supplemented with 3 mg L−1 BAP (Benzylaminopurine), 0.5 mg L−1 IBA (Indole-3-butyric acid), 8 g L−1 agar and 30 g L−1 sucrose (multiplication medium). Regenerated shoots were multiplied on the same medium by sub-culturing at 8 weeks intervals. Eight weeks old micro-shoots from 4th subculture were treated with 50 mg L−1 IBA for 3 h and effect of six different rooting mixes on ex vitro rooting was investigated. All the micro-shoots transferred to the rooting mix consisting of coir dust, soil and sand at 1:1:1 ratio (v/v) (ex vitro rooting mix) survived and produced a well developed root system.