The antibacterial activity of the crude protein extracts was
determined by Agar well diffusion assay (Reeves, 1989).
2.34 g of high sensitivity testing agar was dissolved in 100 ml
of distilled water and autoclaved at 121 C for 15 min. Before
transferring this medium in sterilized petri plates, it was allowed
to cool and then was poured into the petri plates and allowed
to solidify. After this, it is inoculated with activated
culture using sterile cotton swabs. And the wells are created
using sterile agar borer and the wells were filled by adding
25 ll of crude protein extracts and were incubated at 37 C
for 12–24 h. Three replicates were prepared from each sample.
The extracts having antimicrobial activity, inhibit the microbial
growth and the clear zones were formed. The zone of inhibition
was measured in millimeters.