A total of 30 anagen hairs were used for PCR product dilution.
Amplification of the samples was performed on the GeneAmp1
9700 96-well thermal cycler following the AmpFlSTR1 NGM1 kit
manufacturer’s instructions. Each amplified PCR product from a
single anagen hair was diluted into concentrations of 1:10, 1:30
and 1:50, in which 1 mL from a dilution was combined with 9.5 mL
Hi-DiTM Formamide and 0.5 mL LIZ-600TM size standard. Samples
were injected on an Applied Biosystems 3130xl Genetic Analyser at
3 kV for 10 s. The data were analysed using GeneMapper1
software v3.2.