3. Empty the cell, rinse and fill with p-xylene, and run a spectrum on this.
4. Repeat run for o-xylene.
5. From the spectra of the pure substances, indentify the main functional group of xylem compound. Choose a peak of each isomer to measure. Use the baseline method (Figure 3.3) to measure Po/P for the peak of each compound. Prepare a calibration curve of the ratio of log (Po/P)meta / log (Po/P)ortho and log (Po/P)para / log (Po/P)ortho versus concentration for the meta and para isomers, respectively.
6. Run spectra on each of the standard mixture