Forced swimming test (FST)
The FST was performed as described by Porsolt et al. (1979) and carried
out over 2 days, i.e., a day for the pre-swimming session and a day for
the test session. Briefly, in the pre-swimming session, ratswere individually
placed for 15min in open cylinders (45 cmheight × 20 cm diameter)
containing 23 cm of filled water at 25 ± 1 °C. Twenty four hours later,
rats underwent the test session. In the test session, ratswere again placed
in cylinders filledwithwater, and the duration of immobilitywas recorded
during 6min period. Each rat was recorded as immobilewhen floating
motionless or making only those movements necessary to keep its head
above water.
Forced swimming test (FST)The FST was performed as described by Porsolt et al. (1979) and carriedout over 2 days, i.e., a day for the pre-swimming session and a day forthe test session. Briefly, in the pre-swimming session, ratswere individuallyplaced for 15min in open cylinders (45 cmheight × 20 cm diameter)containing 23 cm of filled water at 25 ± 1 °C. Twenty four hours later,rats underwent the test session. In the test session, ratswere again placedin cylinders filledwithwater, and the duration of immobilitywas recordedduring 6min period. Each rat was recorded as immobilewhen floatingmotionless or making only those movements necessary to keep its headabove water.
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