Heterotrophic cultivation is expensive if commercially available organic substrates are used. The use of natural organic compounds can significantly reduce its cost. Literature shows that microalgae species such as C. vulgaris, Scenedesmus acutus, C. protothecoides, Chlorella saccharophila, Chlorella sorokiniana can grow in heterotrophic culture. Contamination is another issue in heterotrophic cultivation [44]. It can be controlled by sterilization and adopting aseptic techniques.