Additionally, the decrease in the activity in the long-chain acyl-CoA oxidases resulted in higher γ-decalactone versus 3-hydroxy-γ-decalactone amounts . 3-hydroxy-γ-decalactone accumulates when NAD supply is not sufficient, and thus, the flux reduction through β-oxidation permits a better supply of the required cofactor to specifically produce γ-decalactone. The production of γ- decalactone was further optimized by Belo et al.