Briefly, competent bacterial
cells were electroporated, according to the protocol of Sambrook
and Russell (2001), in a Gene Pulser II (Bio-Rad) in the presence
of the plasmid pGFPuv in 0.2-cm cuvettes with an electrical pulse
of 4–5 msec at 2.5 kV with 200 X and 25 lF.