The tissues were removed, washed in 0.75% saline solution, blotted with tissue paper, and homogenized in 2 ml of 0.2 M, pH 7.4 phosphate buffer by using the mortar and pestle and finally centrifuged at 8000 rpm for 25 min at 4◦C and the supernatants were taken in Teflon tubes and finally stored at −80◦C for biochemical analysis of amylase, lipase, protease and total protein con-tent.