The experiments were carried out aseptically in
polypropylene beakers covered with microbiological
filter tissue (Lamino Padding Roll, ACCO
®
, Tidi Prod-
ucts Inc.). Each beaker containing 10 g of solid substrate
(dry basis) was sterilised at 111°C for 20 min and then
inoculated with 10
8
spores from spore suspensions pre-
pared in Tween 80 0.1% v
:
v [14]. The moisture content
of the media was adjusted to 70% v
:
w. Cultivation was
carried out at 30°C in an incubator with humidified air
injection, with fermentation times varying from 15 to 65
h.