2.3.1. DPPH radical scavenging activity assay
The DPPH radical scavenging activity of hydrolysates or peptides
was measured using a modified method of Zhang, Li,
Miao, and Jiang (2011). DPPH radical (2 ml 0.1 mM) dissolved
in 95% ethanol was added to 2ml of sample solution. The
mixture was shaken and left for 30 min at room temperature.
Absorbance of the resulting solution was measured at
517 nm. For the blank, 2ml of distilled water were used
instead of the sample. The radical scavenging activity was
calculated as follows:
DPPH scavenging activityð%Þ
¼½ðBlank absorbanceSample absorbanceÞ=Blank absorbance
100:)