Different dilutions of Trolox (200 μM - 12.5 μM) and methanolic mango samples were tested. All solutions were prepared fresh daily. In every working well the following was pipetted in triplicate: Fluorescein, 150 μl of a 10 nM solution; for standard, 25 μl of Trolox dilution; for sample, 25 μl of sample dilution; for blank, 25 μl of phosphate buffer.