Results
Induction of rat model
Body, kidneys and liver’s weigh of rats after treatment with Cd : Body weigh of the control rats gradually increased during the experiment. The body weigh of the rats treated with CdCl_2 did not increase but at the conclusion of the experiment, the mean weights of both group were different (Figure 1). The weights of kidneys and liver’s were not significantly different between the control and the Cd treated rats eventhough the data appeared that Cd treated rats has larger organs than the control rats without CdCl_2 treatment (Table 1).
Blood and urinary Cd in cadmium treated rats : After the Cd exposure, the blood Cd concentration of treated rats was approximately 5,000 times greater than in the control rats (Table 2). Likewise, the urinary Cd concentration in treated rats was much higher than for the control rats.
Histopathological study of cadmium treated rats : The kidneys of the control rats had normal structure of glomerulus (G) and proximal convoluted tubules (T) (Figure 2A). The kidneys of Cd treated rats had proximal renal tubular damage (Figure 2B and 2C). The cuboidal shaped cells were disrupted with undefined epithelial cell lining. The intracellular space or lumen (L) intracellular space or lumen (L) increased. Protein casts (arrow) were present in collecting tubules. Some glomeruli had increased cellular appearance and diminished capsule space.
Histological examination indicated indicated the hepatic architectures in the control group was normal (Figure 3A). The liver of rats exposed to Cd (Figure 3B and 3C) showed degenerative changes like swollen hapatocytes with palecytoplasm and condensed nuclear chromatins with pyknosis. Single cell necrosis (white arrow), marker by contracted cells detached from the others and densely hyperchromatic nuclear chromatins, together with sinusoidal (black arrow) widening was also observed in all animals with Cd treatment.
ResultsInduction of rat model Body, kidneys and liver’s weigh of rats after treatment with Cd : Body weigh of the control rats gradually increased during the experiment. The body weigh of the rats treated with CdCl_2 did not increase but at the conclusion of the experiment, the mean weights of both group were different (Figure 1). The weights of kidneys and liver’s were not significantly different between the control and the Cd treated rats eventhough the data appeared that Cd treated rats has larger organs than the control rats without CdCl_2 treatment (Table 1). Blood and urinary Cd in cadmium treated rats : After the Cd exposure, the blood Cd concentration of treated rats was approximately 5,000 times greater than in the control rats (Table 2). Likewise, the urinary Cd concentration in treated rats was much higher than for the control rats. Histopathological study of cadmium treated rats : The kidneys of the control rats had normal structure of glomerulus (G) and proximal convoluted tubules (T) (Figure 2A). The kidneys of Cd treated rats had proximal renal tubular damage (Figure 2B and 2C). The cuboidal shaped cells were disrupted with undefined epithelial cell lining. The intracellular space or lumen (L) intracellular space or lumen (L) increased. Protein casts (arrow) were present in collecting tubules. Some glomeruli had increased cellular appearance and diminished capsule space. Histological examination indicated indicated the hepatic architectures in the control group was normal (Figure 3A). The liver of rats exposed to Cd (Figure 3B and 3C) showed degenerative changes like swollen hapatocytes with palecytoplasm and condensed nuclear chromatins with pyknosis. Single cell necrosis (white arrow), marker by contracted cells detached from the others and densely hyperchromatic nuclear chromatins, together with sinusoidal (black arrow) widening was also observed in all animals with Cd treatment.
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