Initial enrichment of the sample has been found to yield higher colony count of psychrotolerant bacteria compared to direct plating of samples [35]. Also, use of diluted complex media has been observed to yield higher number of isolates compared to the use of full strength media for isolation [20,33,35]. Minimal media supplemented with amino acids encouraged growth of Antarctic bacterium Pseudomonas syringae Lz4W, and amendment of defined media with yeast extract, peptone or casamino acids significantly improved the growth rate of facultative psychrophilic bacteria Cobetia marina L-2 when the temperature was lowered from 11 to 5°C [11,36]. Therefore, in the present study a minimal media supplemented with filter sterilized peptone as a source of amino acids was used to isolate psychrotolerant bacteria [19].